Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Target Synonym: CKTSF1B1;DAND2;DRM
Protein Tag: N-Trx
Background: GREM1 belongs to the DAN family. It contains 1 CTCK (C-terminal cystine knot-like) domain. GREM1 is a cysteine knot-secreted protein and acts as an inhibitor in the TGF beta signaling pathway. It inhibits BMP-2, -4, and -7. Inhibition by Grem 1 of BMPs in mice allows the expression of fibroblast growth factors (FGFs) 4 and 8 and Sonic hedgehog (Shh) which are necessary for proper limb development. It interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner. As a cytokine, GREM1 may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. It down-regulates the BMP4 signaling in a dose-dependent manner. It also acts as an inhibitor of monocyte chemotaxis. GREM1 is highly expressed in the small intestine, fetal brain, and colon.
Bio Activity: Not validated for activity
Sequence: Met1-Asp184
Purity: > 95% as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Calculated MW: 40.1 kDa
Observed MW: 40 kDa
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