Protein Tag: N-Trx
Uniprot: P16104
Accession: P16104
Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Human Histone H2AX with an N-terminal His tag expressed in E. coli and purified by conventional chromatography.
Bio Acitivity: Not validated for activity
Sequence: Met1-Tyr143
Purity: > 90% as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis
Endotoxin: < 10 EU/mg of the protein as determined by the LAL method
Calculated MW: 35.7 kDa
Observed MW: 35 kDa
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