Recombinant Human MCM3 protein (His tag)

Protein Tag: N-His & C-His

Uniprot: P25205

Accession: P25205

Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with mCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene.

Bio Acitivity: Not validated for activity

Sequence: Ser 41-Ala 300

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin: < 10 EU/mg of the protein as determined by the LAL method.

Calculated MW: 28.5 kDa

Observed MW: 35 kDa

• SKU: ELSPDEH100799-500
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: PDEH100799-500
• Package Unit: 500μg
• Quantity Unit: STK
• Reactivity: Human
• Host: Escherichia Coli