Protein Tag: N-His
Uniprot: Q15063
Accession: Q15063-1
Background: Periostin (POSTN,PN),originally named as osteoblast-specific factor 2 (OSF-2),is a 90-kDa secreted protein which is now classified as a matricellular protein. It is present in a wide variety of normal adult tissues and fetal tissues,and has a role in bone,tooth and heart development and function. Studies show that periostin is overexpressed in a broad range of human cancer types,includidng lung,ovary,breast and colon cancers. Recent evidence reveals that periostin is expressed by fibroblasts in the normal tissue and in the stroma of the primary tumour,and it is required to allow cancer stem cell maintenance.
Bio Acitivity: Not validated for activity
Sequence: Ala21-Leu365
Purity: > 85% as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 20mM Tris,1mM EDTA, pH 8.0
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Endotoxin: < 10 EU/mg of the protein as determined by the LAL method.
Calculated MW: 38 kDa
Observed MW: 40.3 kDa
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