Recombinant Swine IL-4 protein(His Tag)

Protein Tag: C-His

Uniprot: Q04745

Accession: Q04745

Background: Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation; survival and gene expression. IL-4 is produced by mast cells; T cells; and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells; characterized by their cytokine-secretion profile that includes secretion of IL-4; IL-5; IL-6; IL-10; and IL-13; which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.

Bio Acitivity: Measure by its ability to induce proliferation in TF-1 cells.The ED₅₀ for this effect is <0.6 ng/mL.

Sequence: His 25-Cys 133

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 13.5 kDa

Observed MW: 15 kDa

• SKU: ELSPKSS000004-5
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: PKSS000004-5
• Package Unit: 5μg
• Quantity Unit: STK
• Application: Cell Culture