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Applications: Useful for the study the binding of HLA-DR in ELISA and in cellular assays.
Assay Conditions: The antibody was validated by ELISA. HLA-DR protein was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 4 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer and washes, the plate was incubated with Streptavidin-HRP (BioLegend #405210), washed, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.
Background: HLA-DR (human leukocyte antigen -DR isotype) is an MHC (major histocompatibility complex) class II protein and it serves as a ligand for the TCR (T cell receptor) when combined with an immunogenic peptide. Matching HLA isotypes is crucial in organ transplant, with HLA-DR being the main HLA isotype responsible for graft-versus host disease during the first 6 months post-transplant. A number of auto-immune diseases are linked to HLA-DR, including Crohn's disease, lupus, and multiple sclerosis. HLA-DR is found in a subset of NK cells that combine properties from NK and dendritic cells. They are able to produce and release cytokines, degranulate and expand, but also present antigens to CD4+ and CD8+ T cells. The identification of HLA isotypes and understanding of the mechanisms of action of HLA-DR are thus crucial in medicine.
Description: Recombinant human anti-HLA-DR antibody recognizing human HLA-DR (human leukocyte antigen-DR isotype). This anti-HLA-DR antibody is a purified recombinant antibody, which is labeled with biotin using Avi-Tag™ technology at the C-terminus of the heavy chain unit.
Format: Aqueous buffer solution.
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Purity: ≥90%
Storage Stability: At least 12 months at -80°C.
Uniprot: P01903
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
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