Target: CD25.
Clone: PC61.
Specificity: Anti-CD25 In Vivo Antibody - Ultra Low Endotoxin (PC61) recognizes an epitope on Mouse IL-2 RA.
Purification method: This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution: The CD25 antigen is detected on CD4 positive T-cells but not on CD8 positive T-cells. However, when stimulated with phytohemagglutinin (PHA), the CD25 antigen can be detected on CD8 positive T cells. The CD25 antigen is also expressed on CD20 positive B cells. The CD25 antigen is negative on CD 56 positive natural killer cells, CD 14 positive monocytes, granulocytes, red blood cells and platlets. The CD25 antigen is expressed on malignant cells of adult T-cell leukemias (ATL's) carring HTLV-1, most cases of cutaneous T cell lymphoma and lymphoepithelioid lymphomas among non-Hodgkin's T cell lymphomas.
Background: IL-2 receptor alpha chain (IL-2Ralpha) is a component of high-affinity IL-2 receptors and thus is a key regulator of lymphocyte proliferation. IL-2R alpha expression occurs at specific stages of early T and B lymphocyte development and is induced upon activation of mature lymphocytes. IL-2R alpha is essential for regulation of both the size and content of the peripheral lymphoid compartment, probably by influencing the balance between clonal expansion and cell death following lymphocyte activation. IL-2Ralpha is also important in the formation of high-affinity binding sites, and the inducibility of the alpha gene contributes to the highly regulated and transient display of high-affinity IL-2R.
Immunogen: IL-2-dependent cytolytic mouse T-cell clone B6.1.
Concentration: ≥ 2.0 mg/ml.
Formulation: 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Purity: >98% by SDS-PAGE and HPLC.
Endotoxin: ≤ 0.75 EU/mg as determined by the LAL method.
Aggregation: Aggregation level ≤ 1%.
Use: Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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