Cedarlane's anti-rat T cytotoxic/suppressor cell monoclonal antibodies recognize a determinant on the majority of thymocytes (90-95%), a subset of peripheral T cells, the majority of NK cells, and the granular intraepithelial leukocytes in the small intestine (1,2,3,4). The antigen recognized is a complex of surface glycoproteins of Mr 34, 39, and 76 kDa and is the rat homologue of the human CD8 and the mouse Ly 2.3 antigen (5,8). Clone OX-8 labels all peripheral T cells that are unlabelled by clone W3/25 (CL003). It labels a T cell subset which mediates the suppression of antibody formation (1) and the cytotoxic cell precursor (2). OX-8 and W3/25 can be used together to fractionate T cells by sorting in FACS or by rosette depletion (1,2) or can be used together to study subsets of T cells in the rat which mediate lethal graft versus host disease (7). This antibody is one of the 3 monoclonal antibodies which labels T lymphocyte populations in rat, these being clone W3/13 (CL002) which labels all T cells, as well as clones W3/25 and OX-8 which label non-overlapping T cell populations. These monoclonal antibodies used in concert are being employed extensively to investigate cellular aspects of the immune response in rats and prove to be useful as markers for functionally distinct subpopulations of lymphocytes. This clone is reported to work with frozen and paraffin sections (9).
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