Product Description: Caspase 3 antibody detects caspase 3 protein, a 32 kDa zymogen (also known as pro-caspase 3) that is cleaved at conserved aspartic residues into 17 kDa and 12 kDa subunits upon activation. The cleaved subunits then form an active heterotetramer by hydrophobic interactions and trigger subsequent caspase cascades to induce the apoptotic phenotype.
Application Note: WB: 1:500-1:10000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Calculated MW: 32
Positive Control: Jurkat , Raji , NCI-H929 , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 (30uM cisplatin treatment for 24h) , HeLa
Specificity/Sensitivity: IP/MS validation was supported by references (PMID:30377401)
Form: Liquid
Buffer (with preservative): PBS, 1% BSA, 20% Glycerol, 0.025% ProClin 300.
Concentration: 0.15 mg/ml (Please refer to the vial label for the specific concentration.)
Background: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq]
Uniprot ID: P42574
Antigen Species: Human
Immunogen: Recombinant protein encompassing a sequence within the center region of human Caspase 3. The exact sequence is proprietary.
Purification: Purified by antigen-affinity chromatography.
Conjugation: Unconjugated
Full Name: caspase 3
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