Product Description: E-Cadherin antibody detects E-cadherin protein, which is encoded by the CDH1 gene. E-cadherin is a calcium-dependent cell adhesion protein (predicted molecular weight of 97 kDa) that plays a vital role in cell migration and proliferation. Because E-cadherin is an epithelial cell marker, this antibody is a well-published and widely used reagent to study E-cadherin protein expression during the epithelial-mesenchymal transition (EMT) in various physiological and pathological processes.
Application Note: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Calculated MW: 97
Positive Control: MCF-7
Form: Liquid
Buffer (with preservative): PBS, 1% BSA, 20% Glycerol, 0.025% ProClin 300.
Concentration: 0.14 mg/ml (Please refer to the vial label for the specific concentration.)
Background: This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]
Uniprot ID: P12830
Antigen Species: Human
Immunogen: Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.
Purification: Purified by antigen-affinity chromatography.
Conjugation: Unconjugated
Full Name: cadherin 1
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