Clone Name: Hs-8
Application Note: FACS: 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Calculated MW: 44
Form: Liquid
Buffer (with preservative): TBS, 15mM Sodium azide.
Concentration: Batch dependent (Please refer to the vial label for the specific concentration.)
Background: This gene encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. [provided by RefSeq, Jul 2008]
Uniprot ID: O14556
Antigen Species: Human
Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Purification: Purified by size-exclusion chromatography
Conjugation: FITC
Full Name: glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
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