Clone Name: BG5
Product Description: Laminins, which consist of three subunits called alpha, beta and gamma chains, are major cell-adhesive components of extracellular matrix, especially basement membranes (BMs). The laminin family is constituted of over 15 isoforms, and each member is expressed in a tissue-specific manner and plays a differential role in each tissue. In the case of laminin alpha 3 chain, there are two splicing variants, the truncated form alpha 3A and the full-length alpha 3B.. Laminin-3A32(Lm3A32) , formerly called laminin-5 or -5A, is composed of alpha 3A, Beta 3 and gamma 2 chains and distributed in the skin, esophagus, lung, breast and other epithelial tissues. This antibody highly detects laiminin-3A32 in the basement membranes of normal epithelial tissues and of relatively benign or differentiated carcinomas. It may also detect laminin-3A11. As its sister clone F7 is specific for alpha 3B chain, the combination of BG5 and F7 is an important tool to investigate the difference of the full- length and truncated alpha 3 laminins. These antibodies, together with the anti-gamma 2 antibody P2H capable of detecting invasive cancers, are powerful tools in pathological analyses of human cancers.
Application Note: WB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Specificity/Sensitivity: This antibody specifically detects alpha 3A in IHC. However, this clone may cross react with alpha 3B in ELISA, IP and Western Blot.
Form: Liquid
Buffer (with preservative): Ascites, no preservative.
Concentration:
Notes: For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Antigen Species: Human
Immunogen: Recombinant human laminin-alpha 3B N-term ~190 kDa fragment. Alpha 3A chain is truncated form ,and alpha 3B chain is full length.
Purification: Unpurified
Conjugation: Unconjugated
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