p21 Cip1 antibody (GT1032)

p21 Cip1 antibody (GT1032)
SKU
GTX629543-100
Packaging Unit
100 μl
Manufacturer
GeneTex

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Clone Name: GT1032

Product Description: p21 (Cip1) antibody recognizes p21Cip1 protein, a cyclin-dependent kinase inhibitor with a predicted molecular weight of 18 kDa. p21 is a major downstream regulator of p53 activity in response to DNA damage to control cell cycle arrest. p21 also interacts with proliferating cell nuclear antigen (PCNA) to inhibit PCNA-dependent S-phase DNA synthesis.

Application Note: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Calculated MW: 18

Positive Control: HCT116 (30 μM Cisplatin treatment for 24 hr) , HCT116 (30 μM Cisplatin treatment for 48 hr)

Form: Liquid

Buffer (with preservative): PBS, no preservative.

Concentration: 1.45 mg/ml (Please refer to the vial label for the specific concentration.)

Background: This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. [provided by RefSeq]

Uniprot ID: P38936

Antigen Species: Human

Immunogen: Recombinant protein encompassing a sequence within the center region of human p21 Cip1. The exact sequence is proprietary.

Purification: Affinity purified by Protein A.

Conjugation: Unconjugated

Full Name: cyclin dependent kinase inhibitor 1A
More Information
SKU GTX629543-100
Manufacturer GeneTex
Manufacturer SKU GTX629543-100
Package Unit 100 μl
Quantity Unit STK
Reactivity Human, Mouse (Murine)
Clonality Monoclonal
Application Immunofluorescence, Immunohistochemistry (paraffin), Immunoprecipitation, Western Blotting, Flow Cytometry, Immunocytochemistry
Isotype IgG2a
Human Gene ID 1026
Host Mouse
Conjugate Unconjugated
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