Anti-Migis-alpha (29C11)

Anti-Migis-alpha [29C11], Recombinant, IgG1 kappa, Mouse

CloneID: 29C11

Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with a mouse/human chimeric migis-αL-containing protein.

Buffer Composition: PBS only.

Specificity Statement: This antibody binds the extracellular peptide called the Migis-α consisting of 26-32 amino acids towards the N-terminal of the human membrane bound IgA, when the lipid rafts are disrupted.

Application Notes (Clone): Membrane bound IgA is associated with Igα/Igβ as the B cell receptor (BCR) complex on mIgA-expressing B cells. The α chain of mIgA (mα) contains an extracellular domain that is reported to be an antigenic site suitable for isotype-specific targeting of mIgA-expressing B cells by antibodies. The mAb binds strongly to synthetic peptides of migis-α and to various recombinant proteins containing migis-α as revealed by ELISA. Flow cytometric analysis with B cells suggested that the mAb did not bind strongly to mIgA and required a higher concentration for detection. Immunoprecipitation analysis of these mAbs indicated that mIgA could only be pulled down by 29C11 when mIgA-expressing B cells were solubilized by strong detergents, such as sodium dodecyl sulfate (SDS) or when lipid rafts are disrupted (PMID: 21723611).

• SKU: ABAAb02045-1.1-BT
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab02045-1.1-BT
• Package Unit: 1 mg
• Quantity Unit: STK
• Reactivity: Human
• Clonality: Recombinant
• Application: Immunoprecipitation, ELISA
• Isotype: IgG1 kappa
• Host: Mouse