Application Note: This antibody is supplied in 0.01M Sodium Phosphate, 0.25M NaCL, pH: 7.6. Anti-Bovine IgG (heavy and light chain) Antibody is peroxidase conjugated and is suitable for immunoassays where specificity to bovine immunoglobulin is desired. Antibody has been tested by ELISA, western blot immunoblot, and immunohistochemistry. Optimal concentrations in immunoassays should be determined by the researcher.
Concentration Value: 0.8 mg/mL
ELISA Dilution: 1:5,000 - 1:100,000
Immunohistochemistry Dilution: 1:500 - 1:5,000
Western Blot Dilution: 1:5,000 - 1:200,000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: Bovine IgG whole molecule
Physical State: Lyophilized
Purity and Specificity: Anti-Bovine IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Bovine IgG and Bovine Serum.
Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Bovine IgG (H&L) Antibody Peroxidase Conjugated detects Bovine Immunoglobulin G. Immunoglobulin G, IgG, are antibody molecules. Each IgG is a hetereotetramer composed of four peptide chains - two heavy chains and two light chains. Each IgG has two F(ab) antigen binding sites.
Low Endotoxin: No
Secondary Antibody Specificity: IgG (H&L)
Other: User Optimized
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