Elab Fluor® 647 Anti-Mouse CD150/SLAM Antibody(TC15-12F12.2)

Target Synonym: CD 150;Signaling lymphocytic activation molecule;Slam;Slamf1

Usage: Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.

Storage: This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.

Citation Link 1: https://789.bio/ec/HyHm9O

Citation Link 2: C57BL/6 murine splenocytes are stained with FITC Anti-Mouse CD45R/B220 Antibody and Elab Fluor^® 647 Anti-Mouse CD150 Antibody (Left). Splenocytes are stained with FITC Anti-Mouse CD45R/B220 Antibody and Elab Fluor^® 647 Rat IgG2a, λ Isotype Control (Right).

Background: CD150 is a 75-95 kD member of the immunoglobulin superfamily, also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1 but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. In combination with CD48, CD150 is a useful marker for hematopoietic stem cell studies.

Buffer: Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.

• SKU: ELSE-AB-F1177M-50
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: E-AB-F1177M-50
• Package Unit: 50 Tests
• Quantity Unit: PAK
• Reactivity: Mouse (Murine)
• Clonality: Monoclonal
• Application: Flow Cytometry
• Isotype: Rat IgG2a λ
• Human Gene ID: 27218
• Host: Rat
• Conjugate: Conjugated, Elab Fluor647