Elab Fluor® Violet 450 Anti-Mouse CD22 Antibody(Cy34.1)

Target Synonym: Siglec2;B-cell receptor CD22;B-lymphocyte cell adhesion molecule;BL-CAM;CD22;Cd22;Lyb-8;Sialic acid-binding Ig-like lectin 2;Siglec-2;T-cell surface antigen Leu-14

Usage: Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].

Storage: This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.

Citation Link 1: https://789.bio/ec/LCqbDO

Citation Link 2: C57BL/6 murine splenocytes are stained with APC Anti-Mouse CD3 Antibody and Elab Fluor^® Violet 450 Anti-Mouse CD22 Antibody (Left). Splenocytes are stained with APC Anti-Mouse CD3 Antibody and Elab Fluor^® Violet 450 Mouse IgG1, κ Isotype Control (Right).

Background: The Cy34.1 monoclonal antibody specifically binds to the B-lymphocyte differentiation antigen CD22 on strains having the Lyb-8.2 alloantigen (e.g., A, BALB/c, CBA, C3H/He, C57BL, C57L, C58, SJL, SWR, but not AKR, DBA/1, DBA/2, NZB, PL). CD22 is expressed at high levels on mature peripheral B lymphocytes (follicular and marginal zone), B-1 cells (CD5+ B cells), and plasma cells. It is a member of the Ig gene superfamily and associates with the B-cell antigen receptor. Its sialic acid- binding immunoglobulin-like lectin (siglec) extracellular region mediates B-cell adhesion to ligands on endothelial cells in the bone marrow. Its intracellular domain is phosphorylated after cross-linking of antigen receptor or MHC class II antigen. It is involved in negative regulation of B-cell activation and protection from autoimmunity. B-cell proliferative responses to LPS or anti-mouse Ig µ chain are augmented in the presence of Cy34.1 mAb.

Buffer: Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.

• SKU: ELSE-AB-F1021UQ-100
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: E-AB-F1021UQ-100
• Package Unit: 100μg
• Quantity Unit: STK
• Reactivity: Mouse (Murine)
• Clonality: Monoclonal
• Application: Flow Cytometry
• Isotype: Mouse IgG1 κ
• Human Gene ID: 12483
• Host: Mouse
• Conjugate: Conjugated, Elab Fluor Violet 450