Target Synonym: LCA;Ly-5.2
Usage: Each lot of this antibody is quality control tested by flow cytometric analysis.
The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Storage: This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
Citation Link 1:
https://789.bio/ec/SKm14KCitation Link 2:
C57BL/6 murine splenocytes are stained with Elab Fluor® Violet 450 Anti-Mouse CD45.2 Antibody (filled gray histogram) or Elab Fluor® Violet 450 Mouse IgG2a, κ Isotype Control (empty black histogram).Background: CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Buffer: Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
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