Application Note: Golden Syrian Hamster IgG (H&L) Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Concentration Value: 0.5 mg/mL
Flow Cytometry Dilution: 1:100 - 1:250
IF Microscopy Dilution: 1:100 - 1:250
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: Anti-Golden Syrian Hamster IgG (H&L) was produced by repeated immunization with Golden Syrian Hamster IgG whole molecule in rabbit.
Physical State: Lyophilized
Purity and Specificity: F(ab')2 Anti-Golden Syrian Hamster IgG Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian Hamster IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).
Background: F(ab')2 Anti-Golden Syrian Hamster IgG Phycoerythrin Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. Anti-Golden Syrian Hamster IgG (H&L) Antibody is ideal for investigators in Microbiology, Molecular Biology, and Immunology.
Low Endotoxin: No
Secondary Antibody Specificity: IgG (H&L)
Other: FLOW 1:100 - 1:250
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