Application Note: F(ab')2 Anti-Rat IgG Peroxidase Antibody has been tested by ELISA and is assayed against 1.0 µg of Rat IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 of the reconstitution concentration is suggested for this product.
Concentration Value: 1.0 mg/mL
ELISA Dilution: 1:10,000 - 1:100,000
Immunohistochemistry Dilution: 1:500 - 1:2,500
Western Blot Dilution: 1:1,000 - 1:10,000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: Anti-Rat IgG was produced by repeated immunization with rat IgG whole molecule in rabbit.
Physical State: Lyophilized
Purity and Specificity: F(ab')2 Anti-Rat IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
Background: F(ab')2 Anti-Rat IgG (H&L) Antibody generated in rabbit detects specifically rat IgG F(ab')2.F(ab')2 Anti-Rat IgG (H&L) Antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.F(ab')2 Anti-Rat IgG (H&L) Antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.
Low Endotoxin: No
Secondary Antibody Specificity: IgG (H&L)
Other: User Optimized
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