Application Note: Anti-Ferret IgG (gamma chain) antibody is suitable for ELISA, Western Blot and Immunohistochemistry. Antibody should be optimized by end user for reactive conditions.
Concentration Value: 1.0 mg/mL
ELISA Dilution: 1:2,000 - 1:10,000
Immunohistochemistry Dilution: 1:200 - 1:1,000
Western Blot Dilution: 1:500 - 1:2,500
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: Ferret IgG gamma heavy chain
Physical State: Liquid (sterile filtered)
Purity and Specificity: Anti-Ferret IgG (gamma chain) was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Ferret IgG and Ferret Serum. No reaction was observed against Ferret IgA or Ferret IgM. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Ferret heavy or light chain isotypes.
Background: Anti-Ferret IgG Alkaline Phosphatase antibody generated in goat detects ferret IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
Low Endotoxin: No
Secondary Antibody Specificity: IgG (gamma chain)
Other: User Optimized
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