Specificity: Monkey IgG, IgA and IgM, heavy and light chains.
Antibody Modification: HRP
Presentation: Lyophilized from PBS, pH 7.2, No preservatives added
Immunogen: Purified polyclonal monkey IgG, and IgA and IgM containing fractions isolated from monkey serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Resuspension Liquid: 1 ml Distilled water
Gene: Monkey IgG + IgA + IgM
Description: Monkey IgG + IgA + IgM antibody LS-C346633 is an HRP-conjugated goat polyclonal antibody. Validated for DB, ELISA, ICC and IHC.
Usage: The reactivity of the antiserum is directed to the major isotypes of the monkey immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal monkey plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. In enzyme-immunocytochemical and immunohistochemical staining for the detection of cytoplasmic Ig at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500. in ELISA and comparable non-precipitating antibody-binding assays between 1:2000 and 1:10000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Recommended Storage: Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
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