Application Note: WB: 1:5000-1:200000. ELISA: 1:5000-1:100000. IHC: 1:500-1:5000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Specificity/Sensitivity: Pre-adsorbed with Bovine, Horse and Human serum proteins. May react with immunoglobulins from other species.
Form: Liquid
Buffer (with preservative): 10mM Sodium Phosphate, 250mM NaCl, 1.5% BSA, no preservative.
Concentration: 0.8 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species: Rat
Immunogen: Rat IgG F(ab')2 fragment
Target Isotype: IgG F(ab')2
Purification: Purified by antigen-affinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorptions to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation: HRP