Application Note: Anti-Human IgG F(ab')2 fragment has been tested by dot blot and ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Concentration Value: 2.2 mg/mL
ELISA Dilution: 1:14,000 - 1:30,000
Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Western Blot Dilution: 1:2,000 - 1:10,000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: Human IgG F(ab')2 fragment was produced by repeated immunization with Human IgG F(ab')2 fragment in goat.
Physical State: Liquid (sterile filtered)
Purity and Specificity: Anti-Human IgG F(ab')2 (GOAT) was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG, Human IgG F(ab’)2 and Human Serum. No reaction was observed against Human IgG F(c).
Background: Anti-Human IgG F(ab')2 Antibody generated in goat recognizes the dimeric Fab portion of the human IgG molecule. Human IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. F(ab')2 molecules lack the Fc portion of IgG and therefore receptors that bind human IgG F(c) will not bind human IgG F(ab')2 molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Human IgG F(ab')2 fragment antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.
Low Endotoxin: No
Secondary Antibody Specificity: IgG F(ab')2
Other: User Optimized
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