Application Note: Anti-Human κ antibody has been tested by ELISA and western blot and is suitable for immunohistochemistry and immunoassays where specificity to the immunoglobulin kappa subunit is desired. Optimal concentrations in immunoassays should be determined by the researcher.
Concentration Value: 1.0 mg/mL
ELISA Dilution: 1:10,000 - 1:20,000
Immunohistochemistry Dilution: 1:500 - 1:2,000
Western Blot Dilution: 1:1,000 - 1:10,000
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Immunogen: Human kappa light chain
Physical State: Liquid (sterile filtered)
Purity and Specificity: Human κ (kappa chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. Specificity for kappa chain was confirmed by ELISA minimal cross reactivity against other human heavy or lambda light chain isotypes.
Background: The anti-Human κ (kappa chain) Antibody detects the kappa chain subunit. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). In humans the light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain.
Low Endotoxin: No
Secondary Antibody Specificity: κ (kappa chain)
Other: User Optimized
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