Clone: 14A3
Specificity: Recognizes the light chain of duck immunoglobulin, shown to be expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes. Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1, revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+ /14A3+ cells in duck bursa. The recognition of B cells by 14A3 was confirmed using a recently developed Flag-tagged chicken B cell marker, known as chBAFF-Flag (B cell activating factor). Recognizes IgA in Peking duck bile and can be used as a marker to identify B cells in both Peking and Muscovy duck and geese studies. Detects a band of approximately 25 kD in duck cell lysates in Western blotting.
Antibody Modification: Unconjugated
Presentation: PBS, 0.09% Sodium Azide
Immunogen: Purified Peking duck yolk IgY.
Gene: Duck IgY
Description: Duck IgY antibody LS-C58001 is an unconjugated mouse monoclonal antibody. Validated for ELISA, Flow and WB.
Usage: Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.
Recommended Storage: Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Concentration: 1 mg/ml
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