Specificity: Recognizes an epitope in the pFc region of human IgG4. No cross-reactivity is observed with human IgG1, IgG2 or IgG3 isotypes.
Antibody Modification: HRP
Presentation: PBS, pH 7.2, 0.01% Thimerosal
Immunogen: Human IgG4.
Gene: Human IgG4
Description: Human IgG4 antibody LS-C70328 is an HRP-conjugated mouse monoclonal antibody. Validated for ELISA and IHC.
Usage: Suitable for use in ELISA and Immunohistochemistry. ELISA: 1:25600-1:51200. Immunohistochemistry (Frozen sections): 1:40-1:80. Positive control: Tonsil.HRP conjugates are sensitive to light. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Concentration: 1 mg/ml
Recommended Storage: Storage and Stability Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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