Application Note: Anti-Rabbit IgG F(ab')2 fragment has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Concentration Value: 2.0 mg/mL
ELISA Dilution: 1:130,000
Immunohistochemistry Dilution: 1:1,000 - 1:10,000
Western Blot Dilution: 1:2,000 - 1:20,000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: Anti-Rabbit IgG F(ab')2 fragment was produced by repeated immunization with Rabbit IgG F(ab')2 fragment in goat.
Physical State: Lyophilized
Purity and Specificity: Anti-RABBIT IgG F(ab')2 (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab’)2 and Rabbit Serum. No reaction was observed against Rabbit IgG F(c) or Human Serum Proteins.
Background: Anti-Rabbit IgG F(ab')2 fragment antibody generated in goat detects specifically Rabbit IgG F(ab')2 fragment. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Low Endotoxin: No
Secondary Antibody Specificity: IgG F(ab')2
Other: User Optimized
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