Application Note: Anti-Sheep IgG Alkaline Phosphatase Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency.
Concentration Value: 1.0 mg/mL
ELISA Dilution: 1:2,000 - 1:8,000
Immunohistochemistry Dilution: 1:200 - 1:1,000
Western Blot Dilution: 1:1,000 - 1:4,000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: Anti-Sheep IgG (H&L) was produced by repeated immunization with Sheep IgG whole molecule in donkey.
Physical State: Liquid (sterile filtered)
Purity and Specificity: Anti-Sheep IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Sheep IgG and Sheep Serum.
Background: Anti-Sheep IgG (H&L) antibody generated in donkey detects specifically sheep IgG heavy and light chains (H&L). This Anti sheep antibody is conjugated to alkaline phosphatase and this secondary antibody is suitable for immunoblotting and immunoassays.Anti-Sheep IgG (H&L) antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.
Low Endotoxin: No
Secondary Antibody Specificity: IgG (H&L)
Other: User Optimized
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