Shelf life (days): 365.0
Formulation: 50 or 100 µl of protein A-affinity purified monoclonal antibody
Immunogen: Recombinant human CD22
Clone Designation: 340
Target specificity: Human
Cross Reactivity: + CD22
Formula Weight: 0.0
Notes: CD22 is a transmembrane receptor and member of the sialic acid-binding immunoglobulin-type (Ig-type) lectin (SIGLEC) family.{65416,13652} It is composed of N-terminal extracellular Ig-like variable (IgV) and Ig-like constant 2 (IgC2) domains, a membrane-spanning region, and an intracellular immunoreceptor tyrosine-based inhibitory motif (ITIM) domain. CD22 has two isoforms formed via alternative splicing, CD22β, which is the full-length form and contains six IgC2 domains, and CD22α, which lacks the third and fourth IgC2 domains. It is expressed in the cytosol of premature B cells and on the cell surface of resting and activated B lymphocytes but is not expressed in differentiated B cells.{65417} CD22 is an inhibitory receptor activated by binding of α2,6-linked sialic acid-containing molecules, such as glycoproteins, which stimulates phosphorylation of tyrosine in the ITIM domain, leading to recruitment of Src homology 2 domain-containing phosphatases (SHPs), including SHP-1, spleen tyrosine kinase (Syk), LYN, and PI3K.{65416,13652} Knockout of Cd22 decreases IgG1 titers in mice immunized with OVA/alum and expression of human CD22 in Cd22 -/- mice rescues this phenotype.{65418} CD22 is overexpressed in cancer cells isolated from patients with hairy cell leukemia.{65417} Cayman’s CD22 Rabbit Monoclonal Antibody (Clone 340) can be used for ELISA and immunohistochemistry (IHC; paraffin) applications.
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