Recombinant Prokaryotic Lectin-αGal (P. aeruginosa)

Formulation: PBS, pH 7.2

Purity: ≥95% estimated by SDS-PAGE

Shelf life (days): 365

Notes: Recombinant prokaryotic lectin-αGal (RPL-αGal) is a P. aeruginosa lectin expressed in and purified from E. coli. It is a monomer and binds to glycoproteins containing terminal α-linked galactose and/or N-acetylgalactosamine (GalNAc) groups. RPL-αGal activity requires metal ions, therefore, all buffers must be supplemented with 1 mM calcium chloride, magnesium chloride, and manganese chloride. It is recommended to use metal ion-supplemented Tris-buffered saline (TBS), pH 7.6, to dilute RPL-αGal to the desired working concentration (≤20 µg/ml). Following dilution, incubate RPL-αGal for a minimum of 30 minutes prior to use to allow metal complexation.

• SKU: CAY35781
• Manufacturer: Cayman Chemical
• Manufacturer SKU: 35781-2
• Green Labware: No
• Package Unit: 2 mg
• Quantity Unit: STK
• Host: Escherichia Coli