Formulation: PBS, pH 7.2
Purity: ≥95% estimated by SDS-PAGE
Shelf life (days): 365
Notes: Recombinant prokaryotic lectin-Gal2 (RPL-Gal2) is a lectin derived from the P. aeruginosa lectin PA-IL through mutagenesis, then expressed in, and purified from, E. coli. It is a dimer under physiological conditions and selectively binds to glycoproteins containing terminal α-linked galactose groups over glycoproteins containing N-acetylgalactosamine (GalNAc) groups. RPL-Gal2 activity requires metal ions, therefore, all buffers must be supplemented with 1 mM calcium chloride, magnesium chloride, and manganese chloride. It is recommended to use metal ion-supplemented Tris-buffered saline (TBS), pH 7.6, to dilute RPL-Gal2 to the desired working concentration (≤20 µg/ml). Following dilution, incubate RPL-Gal2 for a minimum of 30 minutes prior to use to allow metal complexation.
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