Recombinant Prokaryotic Lectin-Gal4 (P. asymbiotica)

Formulation: PBS, pH 7.2

Purity: ≥95% estimated by SDS-PAGE

Shelf life (days): 365

Notes: Recombinant prokaryotic lectin-Gal4 (RPL-Gal4) is a P. asymbiotica lectin expressed in and purified from E. coli. It is a tetramer under physiological conditions and selectively binds to glycoproteins containing terminal β-linked galactose, N-acetyllactosamine (LacNAc), and Lewis X (LeX) groups. RPL-Gal4 activity requires metal ions, therefore, all buffers must be supplemented with 1 mM calcium chloride, magnesium chloride, and manganese chloride. It is recommended to use metal ion-supplemented Tris-buffered saline (TBS), pH 7.6, to dilute RPL-Gal4 to the desired working concentration (≤20 µg/ml). Following dilution, incubate RPL-Gal4 for a minimum of 30 minutes prior to use to allow metal complexation.

• SKU: CAY36394
• Manufacturer: Cayman Chemical
• Manufacturer SKU: 36394-2
• Package Unit: 2 mg
• Quantity Unit: STK
• Host: Escherichia Coli