Formulation: PBS, pH 7.2
Purity: ≥95% estimated by SDS-PAGE
Shelf life (days): 365
Notes: Recombinant prokaryotic lectin-Sia2 (RPL-Sia2) is a S. gordonii lectin expressed in and purified from E. coli.{65072} It is a monomer under physiological conditions and selectively binds to glycoproteins containing terminal α(2→3)-linked sialic acid (Neu5Ac) groups on O-linked glycans. RPL-Sia2 has been used to detect glycan structures in microarray experiments. RPL-Sia2 activity requires metal ions, therefore, all buffers must be supplemented with 1 mM calcium chloride, magnesium chloride, and manganese chloride. It is recommended to use metal ion-supplemented Tris-buffered saline (TBS), pH 7.6, to dilute RPL-Sia2 to the desired working concentration (≤20 µg/ml). Following dilution, incubate RPL-Sia2 for a minimum of 30 minutes prior to use to allow metal complexation.
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