Notes: 3-azido-7-hydroxy Coumarin is a click-reactive fluorescent dye.{68646,68645} Upon copper(I)-catalyzed azide/alkyne cycloaddition (CuAAC) in a biological system, a fluorescent product is produced that displays excitation/emission maxima of 404 and 477 nM, respectively. 3-azido-7-hydroxy Coumarin has been used for spatially restricted metabolite labeling and the detection of foodborne pathogens.