Product Description: Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide) is a fluorescent dye. Fluorescein-5-maleimide can be used to detect the redox state of thiols in eukaryotic cells. Fluorescein-5-maleimide can label peptides and is used to detect negatively charged nanoparticles. Fluorescein-5-maleimide can also label actin to explore its interaction with cardiac myosin-binding protein C (cMyBP-C), which helps in developing small molecule modulators for heart failure. Fluorescein-5-maleimide can screen mutant proteins that contain cysteine residues. The excitation wavelength of Fluorescein-5-maleimide is 494 nm, and the emission wavelength is 519 nm[1][2][3][4].
Formula: C24H13NO7
References: [1]Wadley AJ, et al. Detecting intracellular thiol redox state in leukaemia and heterogeneous immune cell populations: An optimised protocol for digital flow cytometers. MethodsX. 2018 Nov 1;5:1473-1483. /[2]Akinloye O, et al. Peptide-based fluorescence biosensors for detection/measurement of nanoparticles. Anal Bioanal Chem. 2017 Feb;409(4):903-915. /[3]Bunch TA, et al. Drug discovery for heart failure targeting myosin-binding protein C. J Biol Chem. 2023 Dec;299(12):105369. /[4]McLachlin DT, et al. A method of screening for mutant proteins containing cysteine residues using fluorescein-5-maleimide. Protein Expr Purif. 1996 May;7(3):275-80.
CAS Number: 75350-46-8
Molecular Weight: 427.36
Compound Purity: 99.05
Research Area: Others
Solubility: DMSO : 50 mg/mL (ultrasonic)
Target: Fluorescent Dye
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