Caspase 9 Activity Assay Kit(Colorimetric Method)

Minimum Order Value: 250€

Tested Sample Types: Cell and tissue samples

Type: Apoptosis/Caspase

Assay Time: 4-6 hours

Detection Type: Colorimetric method

Test Principle: Caspase 9, also known as ICE-LAP6 or Mch6, is a relatively upstream caspase in the process of apoptosis signal transduction. After mitochondria release cytochrome c, caspase 9 can form complex with cytochrome c and Apaf1 and can be activated. Activated caspase 9 can activate caspase 3, the most critical enzyme of apoptosis, thereby promoting subsequent apoptotic signals. The activation of caspase 9 can be regulated by phosphorylation. This caspase 9 activity assay kit is based on caspase 9 that can catalyze the substrate Ac-LEHD-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 9 can be calculated by determining the absorbance at 405 nm.

Citation Link 1: https://www.sciencedirect.com/science/article/pii/S0141813025006841

Citation Link 2: https://www.sciencedirect.com/science/article/pii/S0925521423003162

Citation Link 3: https://www.sciencedirect.com/science/article/pii/S0925521423001369

• SKU: ELSE-CK-A389-20
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: E-CK-A389-20
• Package Unit: 20 Assays
• Quantity Unit: PAK