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Application: Measuring AAV2 titer in purified samples.
Background: Recombinant adeno-associated virus (rAAV) is a widely used vector in gene therapy applications. rAAVs exist in the nucleus as episomes and are unable to integrate into the cellular genome. While this brings safety benefits, as there is no risk of random integration and activation of oncogenes, the cell division rate dictates the half-life for transgene expression. Their application is thus particularly useful for the delivery of transgenes to post-mitotic tissues like the brain, retinal, liver, skeletal muscle, and the heart. The payload carried by AAVs is somewhat smaller than other viral tools used for transduction, usually smaller than 5 kb. There are several serotypes, each one with a specific tissue tropism. During production, AAV titers can be measured as a physical titer, infectious titer, or ratio of full to empty capsids. Accurately determining the titer is essential not only for setting clinical doses but also as a foundational step in various analytical assays for characterizing AAV products. The use of an ELISA-based assay to measure AAV2 particles allows for better inter- and intra-laboratory consistency, compared to the use of primers in qPCR. This is a well-suited kit for adoption in gene therapy research and can be further automated for high-throughput applications.
Contraindications: This assay kit is compatible with up to 1% final DMSO concentration.
Description: AAV2 Chemiluminescence ELISA Titration Kit is a sandwich ELISA (Enzyme-linked immunosorbent assay) designed to detect and quantify AAV2 (adeno-associated virus type 2) non-denatured capsids. It provides accurate, specific, and sensitive assessment of the number of AAV2 capsids (full and empty) present in purified AAV2 samples. This kit contains all the materials necessary for 100 reactions, including high sensitivity ELISA ECL reagents, and this kit has a linear range between 5x107 -1x1010 capsids/ml. Note: The capture and detection antibodies detect a conformational epitope not present on unassembled capsid proteins.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Target: AAV2
Warnings: Avoid freeze/thaw cycles. This product is for research purposes only and not for human or therapeutic use. This product should be considered hazardous and is harmful by inhalation, in contact with skin, eyes, clothing, and if swallowed. If contact occurs, wash thoroughly.
Biosafety Level: BSL-1
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