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Applications: Screen small molecule inhibitors in high throughput screening (HTS) applications.
Background: Cathepsin V, also called Cathepsin L2, is a lysosomal cysteine endopeptidase with high sequence homology to cathepsin L and other members of the papain superfamily of cysteine proteinases. Its expression is regulated in a tissue-specific manner and is high in thymus, heart, brain, testis, and cornea. It is involved in maturation of MHC class II molecules, turnover of elastin and breakdown of intra and extra-cellular substrates. Expression analysis of cathepsin V in human tumors revealed widespread expression in colorectal and breast carcinomas, suggesting a possible role in tumor processes. It has also been linked to atherosclerosis, hypertension, and aortic aneurysm, amongst other vascular-related pathologies. Further studies into its functions will allow us to understand in depth the potential benefits of targeting Cathepsin V for therapeutic purposes.
Description: The Cathepsin V Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin V for screening and profiling applications. The Cathepsin V assay kit comes in a convenient 96-well or 384-well format, with enough purified Cathepsin V (amino acids 18-334), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control.The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The Cathepsin V assay kit comes in a convenient 96-well format, with purified Cathepsin V, its fluorogenic substrate, and Cathepsin buffer for 100 enzyme reactions.
Uniprot: O60911
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Lecaille F., et al., 2022 Molecular Aspects of Medicine 88: 101086.
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