PADI4 Fluorogenic Assay Kit

PADI4 Fluorogenic Assay Kit
SKU
BPS82111
Packaging Unit
96 reactions
Manufacturer
BPS Bioscience

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Applications: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Background: PADI4, also known as peptidylarginine deiminase 4, is a hydrolase that catalyzes the conversion of peptidylarginine to citrulline, a process known as citrullination. PADI4 plays a role in several cellular functions, including gene expression, cell differentiation, and apoptosis. It is involved in immune responses and inflammation through granulocyte and macrophage development and thus contributes to diseases such as RA (rheumatoid arthritis). Dysfunction of PADI4 has also been linked to multiple sclerosis (MS), Alzheimer ʹs disease (AD)  and cancer. It contributes to cancer by several possible mechanisms such as epithelial-to-mesenchymal transition (EMT), apoptosis, and formation of neutrophil extracellular traps (NETs). A better understanding of the contribution of PADI4 to cancer development and inflammation, combined with development of inhibitors, will result in new therapeutic avenues.

Contraindications: The final concentration of DMSO in the assay should not exceed 1%. 

Description: The PADI4 Fluorogenic Assay Kit is a fluorogenic assay designed to measure the enzymatic activity of PADI4 (protein-arginine deiminase type-4) for screening and profiling applications. This assay uses o-phthalaldehyde and DTT (dithiothreitol) as detection reagents. The assay kit comes in a convenient 96-well format, with enough purified recombinant PADI4, BAEE (Nα-Benzoyl-L-arginine ethyl ester hydrochloride), EDTA (Ethylenediaminetetraacetic acid), o-phthalaldehyde, and assay buffer for 100 reactions.

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Uniprot: Q9UM07

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

References: Wu X., et al., 2023 Anticancer Agents Med Chem 23(3): 256-265
More Information
SKU BPS82111
Manufacturer BPS Bioscience
Manufacturer SKU 82111
Green Labware No
Package Unit 96 reactions
Quantity Unit PAK
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