Human bFGF ELISA Kit

Shelf Life: 6 months from date of receipt

Uniprot ID: P09038

Background: FGF2 is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.

Assay Range: 102.4 pg/ml - 10,000 pg/ml

Assay Type: Sandwich ELISA

Detection Method: Biotinylated-Streptavidin-HRP

Sample Type: serum_plasma_cell_culture_supernatant

Contents:

• Human bFGF Pre-Coated 96-well Strip Plate, 1 each
• Human bFGF Standard vial, 2 each
• Human bFGF Detection Antibody
• Assay Diluent(s)
• Streptavidin-HRP Solution, 200µl
• TMB Substrate, 12 ml
• Stop Solution, 12 ml
• 20X Wash Buffer, 25 ml
• Sealing Tape, 2 sheets

• SKU: BEYE82-024
• Manufacturer: Bethyl Laboratories
• Manufacturer SKU: E82-024
• Package Unit: 96 wells
• Quantity Unit: STK
• Reactivity: Human
• Application: ELISA
• Human Gene ID: 2247