Human IL-12 p70 ELISA Kit

Shelf Life: 6 months from date of receipt

Uniprot ID: P29459

Background: IL12A encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.

Assay Range: 0.8 pg/ml - 600 pg/ml

Assay Type: Sandwich ELISA

Detection Method: Biotinylated-Streptavidin-HRP

Sample Type: serum_plasma_cell_culture_supernatant

Contents:

• Human IL-12 p70 Pre-Coated 96-well Strip Plate, 1 each
• Human IL-12 p70 Standard vial, 2 each
• Human IL-12 p70 Detection Antibody
• Assay Diluent(s)
• Streptavidin-HRP Solution, 200µl
• TMB Substrate, 12 ml
• Stop Solution, 12 ml
• 20X Wash Buffer, 25 ml
• Sealing Tape, 2 sheets

• SKU: BEYE82-013
• Manufacturer: Bethyl Laboratories
• Manufacturer SKU: E82-013
• Package Unit: 96 wells
• Quantity Unit: STK
• Reactivity: Human
• Application: ELISA
• Human Gene ID: 3592