Human IL-17F(Interleukin 17F) ELISA Kit

Storage: 2-8°C/-20°C,12 months

Target Synonym: ML1;ML-1;IL-17F;IL17F;Cytokine ML-1

Usage: This ELISA kit applies to the in vitro quantitative determination of Human IL-17F concentrations

Type: Sandwich-ELISA

Assay Time: 3 h 30 min

Detection Type: Colorimetric method;ELISA;Sandwich

Sensitivity: 9.38 pg/mL

Detection Range: 15.63-1000 pg/mL

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-17F. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-17F and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-17F, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-17F. You can calculate the concentration of Human IL-17F in the samples by comparing the OD of the samples to the standard curve.

• SKU: ELSE-EL-H4193-96
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: E-EL-H4193-96
• Package Unit: 96 T
• Quantity Unit: PAK
• Application: ELISA