Shelf Life: 6 months from date of receipt
Protein Ref Seq ID: NP_000580.1
Uniprot ID: P05112
Background: IL-4 is a pleiotropic cytokine produced by activated T cells. It acts by binding to the interleukin 4 receptor and STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. IL-4 is considered an important cytokine for tissue repair, counterbalancing the effects of proinflammatory type 1 cytokines, however, it also promotes allergic airway inflammation. Moreover, IL-4, a type 2 cytokine, mediates and regulates a variety of human host responses such as allergic, anti-parasitic, wound healing, and acute inflammation. In an allergic response, IL-4 has an essential role in the production of allergen-specific immunoglobin (Ig) E. [provided by RefSeq, Aug 2020] [taken from NCBI Entrez Gene (Gene ID: 3565)].
Assay Range: 3.13 pg/ml - 200 pg/ml
Assay Type: Sandwich ELISA
Detection Method: Biotinylated-Streptavidin-HRP
Sample Type: serum_plasma_cell_culture_supernatant
Contents:
- Human IL-4 Pre-Coated 96-well Strip Plate, 1 each
- Human IL-4 Standard vial, 2 each
- Human IL-4 Detection Antibody
- Assay Diluent(s)
- Streptavidin-HRP Solution, 200µl
- TMB Substrate, 12 ml
- Stop Solution, 12 ml
- 20X Wash Buffer, 25 ml
- Sealing Tape, 2 sheets
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