Human IL-5 ELISA Kit

Shelf Life: 6 months from date of receipt

Protein Ref Seq ID: NP_000870.1

Uniprot ID: P05113

Background: IL-5 is a homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. IL-5 also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation. IL-5 exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P05113].

Assay Range: 2.74 pg/ml - 2000 pg/ml

Assay Type: Sandwich ELISA

Detection Method: Biotinylated-Streptavidin-HRP

Sample Type: serum_plasma_cell_culture_supernatant

Contents:

• Human IL-5 Pre-Coated 96-well Strip Plate, 1 each
• Human IL-5 Standard vial, 2 each
• Human IL-5 Detection Antibody
• Assay Diluent(s)
• Streptavidin-HRP Solution, 200µl
• TMB Substrate, 12 ml
• Stop Solution, 12 ml
• 20X Wash Buffer, 25 ml
• Sealing Tape, 2 sheets

• SKU: BEYE82-011
• Manufacturer: Bethyl Laboratories
• Manufacturer SKU: E82-011
• Package Unit: 96 wells
• Quantity Unit: STK
• Reactivity: Human
• Application: ELISA
• Human Gene ID: 3567