Human PDGF-BB ELISA Kit

Shelf Life: 6 months from date of receipt

Uniprot ID: P01127

Background: PDGFB is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

Assay Range: 0.549 pg/ml - 400 pg/ml

Assay Type: Sandwich ELISA

Detection Method: Biotinylated-Streptavidin-HRP

Sample Type: serum_plasma_cell_culture_supernatant

Contents:

• Human PDGF-BB Pre-Coated 96-well Strip Plate, 1 each
• Human PDGF-BB Standard vial, 2 each
• Human PDGF-BB Detection Antibody
• Assay Diluent(s)
• Streptavidin-HRP Solution, 200µl
• TMB Substrate, 12 ml
• Stop Solution, 12 ml
• 20X Wash Buffer, 25 ml
• Sealing Tape, 2 sheets

• SKU: BEYE82-021
• Manufacturer: Bethyl Laboratories
• Manufacturer SKU: E82-021
• Package Unit: 96 wells
• Quantity Unit: STK
• Reactivity: Human
• Application: ELISA
• Human Gene ID: 5155