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Application: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.
Background: KAT1 (lysine acetyltransferase 1), also known, HAT1 (histone acetyltransferase 1), is a type B HAT, a type that is mainly cytoplasmic. It regulates chromatin structure, mitochondrial function, and DNA repair. HAT1 acetylates newly synthesized histones in the cytoplasm and succinylates histones and non-histone proteins. HAT1 also plays a role in the immune system, as it was shown that HAT1 is involved in the acetylation of PLZF (promyelocytic leukemia zinc zinger) and thus participates in regulating NF-κB (nuclear factor kappa-light-chain enhanced of activated B cells). The epigenetic regulation activity of this protein is involved in a wide variety of diseases such as cancer, inflammatory diseases, and viral infections. In the context of cancer HAT1 seems to contribute on multiple fronts, by regulating glucose metabolism, DNA damage repair (DDR), cell proliferation. The development of inhibitors targeting HAT1 may open new therapeutic avenues in those disorders.
Contraindications: This assay kit is compatible with up to 1% final DMSO concentration.
Description: The KAT1 (HAT1) Chemiluminescent Assay Kit is an ELISA-based assay designed to measure the histone acetyltransferase activity of HAT1 (histone acetyltransferase 1, also known as KAT1) for screening and profiling applications. This kit comes in a convenient 96-well format with enough purified KAT1, acetyl donor, pre-coated plate with histone substrate, all the reagents necessary for assay detection, and blocking buffer for 96 enzyme reactions. Figure 1. KAT1 (HAT1) Chemiluminescent Assay Kit schematic. KAT1 protein is added to a plate pre-coated with a histone substrate in the presence of acetyl-CoA. After washing, an anti-acetyl-lysine antibody is added, followed by a secondary HRP-antibody. Lastly, HRP substrate is added and the chemiluminescence signal generated can be measured. The signal is proportional to the acetyltransferase activity of KAT1.Need us to run inhibitor screens or profile your compounds against KAT1? Check out our Acethyltransferase Screening &Profiling Services.
Format: Catalog #Name102176HAT1 (KAT1), FLAG-Tag*52028Histone H4, Full Length, His-Tag*829461 mM Acetyl-CoA 797082x HAT Assay Buffer52100Blocking Buffer 482966Primary Antibody 3452131HSecondary HRP-Labeled Antibody 2827350.5M DTT79670ELISA ECL Substrate A (translucent bottle)ELISA ECL Substrate B (brown bottle)79699White 96-well plate *The concentration of the protein is lot-specific and will be indicated on the tube.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: This kit comes in a convenient 96-well format with enough purified KAT1, acetyl donor, pre-coated plate with histone substrate, all the reagents necessary for assay detection, and blocking buffer for 96 enzyme reactions.
Target: KAT1
Uniprot: O14929
Unit Definition: Assay Kit
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Poziello A., et al., 2021 Epigenetics 16 (8): 838-850.
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