Myeloperoxidase Peroxidation Fluorometric Assay Kit

Shelf life (days): 365.0

Formula Weight: 0.0

Notes: Cayman’s MPO Peroxidation Fluorometric Assay provides a convenient fluorescence-based method for detecting the MPO peroxidase activity in both crude cell lysates and purified enzyme preparations. The assay utilizes the peroxidase component of MPO, where a single two electron oxidation of native enzyme (MPO) to compound I (MPO-I) is followed by two successive one electron reductions back to native enzyme by compound II (MPO-II).7 The reaction between hydrogen peroxide and ADHP (10-acetyl-3,7-dihydroxyphenoxazine) produces the highly fluorescent compound resorufin (See Figure 1). Resorufin fluorescence can be easily analyzed with an excitation wavelength of 530-540 nm and emission wavelength of 585-595 nm. The kit includes a MPO-specific inhibitor for distinguishing between MPO activity from MPO-independent fluorescence.

• SKU: CAY700160-192
• Manufacturer: Cayman Chemical
• Manufacturer SKU: 700160-192
• Package Unit: 192 Well
• Quantity Unit: PAK
• Application: Fluorescence