QuicKey Pro Mouse IL-18 (Interleukin 18) ELISA Kit

Storage: 2-8°C，6 months

Target Synonym: IL18;IGIF;IL-1g;IL-1F4;Interferon-gamma-inducing factor

Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse IL-18 concentrations in serum, plasma and other biological fluids.

Tested Sample Types: Serum, plasma and other biological fluids

Type: Sandwich-ELISA

Assay Time: 1 h 30 min

Detection Type: Colorimetric method;ELISA;Sandwich

Sensitivity: 0.09 ng/mL

Detection Range: 0.16-10 ng/mL

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-18. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IL-18 are added to the micro ELISA plate wells. Mouse IL-18 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse IL-18.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-18. The concentration of Mouse IL-18 in the samples is then determined by comparing the OD of the samples to the standard curve.

• SKU: ELSE-OSEL-M0020-48
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: E-OSEL-M0020-48
• Package Unit: 48 T
• Quantity Unit: PAK
• Application: ELISA