THUNDER Phospho-4EBP1 (T37/T46) + Total 4EBP1 TR-FRET Cell Signaling Assay Kit

Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-4EBP1 (T37/T46) and Total 4EBP1 in cell lysates.

Components: Eu-phospho-4EBP1 (20 µL), Acc-phospho-4EBP1 (80 µL), Eu-Total 4EBP1 (5 µL), Acc-Total 4EBP1 (20 µL), 4EBP1 control lysate (500 µL), 5X Lysis Buffer 2 (5 mL), 10X TR-FRET Detection buffer (250 µL), 100X Phosphatase Inhibitor Cocktail (250 µL).

Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.

Assay Format: Half-area 96-well or low-volume 384-well plate.

Assay Target: Phospho-4EBP1 (T37/T46) & Total 4EBP1.

Assay Target Class: Kinase.

SwissProt: Q13541.

Detection method: TR-FRET.

Sample type: Cell lysates (adherent or suspension cells).

• SKU: BIXKIT-4EBP1PT-500
• Manufacturer: BioAuxilium
• Manufacturer SKU: KIT-4EBP1PT-500
• Package Unit: 500 Points (400 Points for phospho; 100 Points for total)
• Quantity Unit: PAK
• Reactivity: Human
• Application: Cell-based Assay
• Human Gene ID: 1978